Biology | Biology homework help

Gene Expression

Label the figure below:

What are the THREE differences between DNA and RNA?

What are the THREE types of RNA? Describe the function of each.

This is considered the

of molecular biology.

Replicate this DNA strand: (Replication Fork) 3’ – TACCCCATAGGATTCACT – 5’ (Origin of Replication)

Where in the cell is replication happening?

Will the replicated strand (the one you wrote) have Okazaki fragments? Explain your answer.

Which strand of the DNA will serve as the template for transcription?

What are the steps of transcription?

Where in the cell is transcription happening?

Write out the sequence of the template strand of DNA. This is just to assist you with the next question �

Transcribe mRNA from the template strand of DNA.

What must be done to the RNA to be ready for translation?

Translate the RNA into protein (use the chart below).

Where in the cell is translation happening?

Mutations

Part 1. Vocabulary

Directions: Define the following terms:

Vocabulary Term Definition

Mutation

Point Mutation

Frameshift Mutation

Missense Mutation

Silent Mutation

Nonsense Mutation

Part 2. Practice

Directions: Complete the boxes below by finding the mRNA and amino acid sequence. Compare the mutant
DNA strands to the original strand. Classify each mutation.

Original DNA sequence: T A C G C G T G C A C G A T G C A G T A G T A C

mRNA sequence:

amino acid Sequence:

Mutation #1 DNA sequence: T A C G C G T G C A C G A T C C A G T A G T A C

mRNA sequence:

amino acid Sequence:

Type of Mutation:

Mutation #2 DNA sequence: T A C G C G T G C T C G A T G C A G T A G T A C

mRNA sequence:

amino acid Sequence:

Type of Mutation:

Mutation #3 DNA sequence: T A C G C G C T G C A C G A T G C A G T A G T A C

mRNA sequence:

amino acid Sequence:

Type of Mutation:

Part 3. Cystic Fibrosis
Directions: Cystic Fibrosis is a disorder where the individuals have lung and kidney problems. The disorder is
caused by a mutation in one of the individual’s genes. Complete the boxes below by finding the mRNA and
amino acid sequence. Compare the mutant DNA strands to the original strand. Classify each mutation.

Part 4. Sickle Cell
Below are the DNA sequence for the normal hemoglobin protein and the mutant DNA sequence for the sickle
cell hemoglobin protein.

Normal Hemoglobin: G G G C T T C T T T T T
Sickle Cell Hemoglobin: G G G C A T C T T T T T

A. Transcribe and translate the normal and sickle cell DNA.

B. Identify this type of mutation and explain why it is

C. If the mutant DNA sequence was G G G C T T C T T A A A , would this mutant DNA result in sickle cell

hemoglobin? Explain your answer.

2. Mutant Gene 1: A C C A T G A A A G A A A A T A T A A T A T T G G T G T T T C C T A T G A T

mRNA sequence:

Amino acid sequence:

Type of Mutation:

3. Mutant Gene 2: A C C A T G A A A G A A A A A A T A A T A T T T G G T G T T T C C T A T G A T

mRNA sequence:

Amino acid sequence:

Type of Mutation:

1. Normal Gene: A C C A T G A A A G A A A A T A T A A T A T T T G G T G T T T C C T A T G A T

mRNA sequence:

Amino acid sequence:

NORMAL mRNA sequence:

NORMAL Amino acid sequence:

MUTANT mRNA sequence:

MUTANT Amino acid sequence:

  • Learning Activity – Gene Expression formatted.pdf
  • Mutations.pdf
    • Part 2. Practice
    • Part 3. Cystic Fibrosis
    • Part 4. Sickle Cell
      • Normal Hemoglobin: G G G C T T C T T T T T Sickle Cell Hemoglobin: G G G C A T C T T T T T
      • B. Identify this type of mutation and explain why it is
      • C. If the mutant DNA sequence was G G G C T T C T T A A A , would this mutant DNA result in sickle cell hemoglobin? Explain your answer.
  1. This is considered the:
  2. of molecular biology:
  3. undefined:
  4. undefined_2:
  5. undefined_3:
  6. undefined_4:
  7. undefined_5:
  8. undefined_6:
  9. Text1:
  10. Text2:
  11. Text3:
  12. Text4:
  13. Text5:
  14. Text6:
  15. Text7:
  16. Text8:
  17. Text9:
  18. Text10:
  19. Text11:
  20. Text12:
  21. Text13:
  22. DefinitionMutation:
  23. DefinitionPoint Mutation:
  24. DefinitionFrameshift Mutation:
  25. DefinitionMissense Mutation:
  26. DefinitionSilent Mutation:
  27. DefinitionNonsense Mutation:
  28. mRNA sequence:
  29. amino acid Sequence:
  30. Mutation 1 DNA sequence T A C G C G T G C A C G A T C C A G T A G T A C:
  31. amino acid Sequence_2:
  32. Type of Mutation:
  33. Mutation 2 DNA sequence T A C G C G T G C T C G A T G C A G T A G T A C:
  34. amino acid Sequence_3:
  35. Type of Mutation_2:
  36. Mutation 3 DNA sequence T A C G C G C T G C A C G A T G C A G T A G T A C:
  37. amino acid Sequence_4:
  38. Type of Mutation_3:
  39. mRNA sequence_2:
  40. Amino acid sequence:
  41. mRNA sequence_3:
  42. Amino acid sequence_2:
  43. Type of Mutation_4:
  44. mRNA sequence_4:
  45. Amino acid sequence_3:
  46. Type of Mutation_5:
  47. NORMAL mRNA sequence:
  48. NORMAL Amino acid sequence:
  49. MUTANT mRNA sequence:
  50. MUTANT Amino acid sequence:
  51. Text14:
  52. Text15:
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