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Presentation/PPT
27/08/2022
Gene Expression
Label the figure below:
What are the THREE differences between DNA and RNA?
What are the THREE types of RNA? Describe the function of each.
This is considered the
of molecular biology.
Replicate this DNA strand: (Replication Fork) 3’ – TACCCCATAGGATTCACT – 5’ (Origin of Replication)
Where in the cell is replication happening?
Will the replicated strand (the one you wrote) have Okazaki fragments? Explain your answer.
Which strand of the DNA will serve as the template for transcription?
What are the steps of transcription?
Where in the cell is transcription happening?
Write out the sequence of the template strand of DNA. This is just to assist you with the next question �
Transcribe mRNA from the template strand of DNA.
What must be done to the RNA to be ready for translation?
Translate the RNA into protein (use the chart below).
Where in the cell is translation happening?
Mutations
Part 1. Vocabulary
Directions: Define the following terms:
Vocabulary Term Definition
Mutation
Point Mutation
Frameshift Mutation
Missense Mutation
Silent Mutation
Nonsense Mutation
Part 2. Practice
Directions: Complete the boxes below by finding the mRNA and amino acid sequence. Compare the mutant
DNA strands to the original strand. Classify each mutation.
Original DNA sequence: T A C G C G T G C A C G A T G C A G T A G T A C
mRNA sequence:
amino acid Sequence:
Mutation #1 DNA sequence: T A C G C G T G C A C G A T C C A G T A G T A C
mRNA sequence:
amino acid Sequence:
Type of Mutation:
Mutation #2 DNA sequence: T A C G C G T G C T C G A T G C A G T A G T A C
mRNA sequence:
amino acid Sequence:
Type of Mutation:
Mutation #3 DNA sequence: T A C G C G C T G C A C G A T G C A G T A G T A C
mRNA sequence:
amino acid Sequence:
Type of Mutation:
Part 3. Cystic Fibrosis
Directions: Cystic Fibrosis is a disorder where the individuals have lung and kidney problems. The disorder is
caused by a mutation in one of the individual’s genes. Complete the boxes below by finding the mRNA and
amino acid sequence. Compare the mutant DNA strands to the original strand. Classify each mutation.
Part 4. Sickle Cell
Below are the DNA sequence for the normal hemoglobin protein and the mutant DNA sequence for the sickle
cell hemoglobin protein.
Normal Hemoglobin: G G G C T T C T T T T T
Sickle Cell Hemoglobin: G G G C A T C T T T T T
A. Transcribe and translate the normal and sickle cell DNA.
B. Identify this type of mutation and explain why it is
C. If the mutant DNA sequence was G G G C T T C T T A A A , would this mutant DNA result in sickle cell
hemoglobin? Explain your answer.
2. Mutant Gene 1: A C C A T G A A A G A A A A T A T A A T A T T G G T G T T T C C T A T G A T
mRNA sequence:
Amino acid sequence:
Type of Mutation:
3. Mutant Gene 2: A C C A T G A A A G A A A A A A T A A T A T T T G G T G T T T C C T A T G A T
mRNA sequence:
Amino acid sequence:
Type of Mutation:
1. Normal Gene: A C C A T G A A A G A A A A T A T A A T A T T T G G T G T T T C C T A T G A T
mRNA sequence:
Amino acid sequence:
NORMAL mRNA sequence:
NORMAL Amino acid sequence:
MUTANT mRNA sequence:
MUTANT Amino acid sequence:
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